Animal Cell Biotechnology: Methods and Protocols (Methods in Molecular Biology) 3rd ed
Mammalian cells are used in industry as well as in research for a variety of applications. Examples are the production of monoclonal antibodies or proteins for diagnostic or therapeutic use, production of viral vaccines as well as cultivation of tissue cells for artifi cial organs or for gene therapy. Besides the techniques required for establishing specific cell lines, techniques are required for optimized cultivation in small and large scale, cell characterization and analysis, purification of biopharmaceuticals and vaccines.
Animal Cell Biotechnology: Methods and Protocols constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines for production of biopharmaceuticalsmand optimizing critical parameters for cell culture considering the whole cascade from the lab to the fi nal production. Scientists with long-refi ned expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line development and selection, protein expression, optimizing their growth in defi ned media, operating the fermentation at high time-space-yield, and in analysis. Topics include basic techniques for establishment of production cell lines, transient protein expression, techniques for optimization of process parameters, cell characterization and analysis, productionscale cultivation techniques, advanced applications of biopharmaceuticals and vaccines, and finally legislatory issues. The volume is divided into fi ve parts that refl ect the processes required for different stages of production.
In Part I, basic techniques for establishment of production cell lines are addressed, especially high-throughput synchronization, insect cell lines, transient gene and protein expression, DNA Profiling and Characterization.
Part II addresses tools for process and medium optimization as well as microcarrier technology.
Part III covers monitoring of cell growth, viability and apoptosis, metabolic flux estimation, quenching methods as well as NMR-based techniques.
Part IV details cultivation techniques, such as microfl uidic perfusion culture, disposable bioreactors, confi guration of bioreactors, spectroscopic and microscopic techniques as well as seed train optimization.
Part V describes special applications, including vaccine production, baculovirus protein expression, chromatographic techniques for downstream as well as membrane techniques for virus separation.
In summary, this volume constitutes a comprehensive manual of state-of-the-art and new techniques for setting up mammalian cell lines for production of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Inevitably, some omissions will occur in the test, but the authors have sought to avoid duplications by extensive cross-referencing to chapters in other volumes of this series and elsewhere. We hope the volume provides a useful compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes. The editor is grateful for the support of all the contributors, the series editor Prof. John Walker, Hertfordshire , UK and the publishers who have made this volume possible.
Hamburg , Germany Ralf Pörtner
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